Tang Prize in Biopharmaceutical Science honors three scientists for developing COVID-19 mRNA vaccines

Tang Prize Laureates

To promote worldwide visibility of the IUBMB, the Tang Prize Foundation, an international prize and education foundation, is invited to provide financial support for major undertakings of the IUBMB. The Tang Prize in Biopharmaceutical Sciences continue to garner much attention worldwide. This year’s award went to three scientists for developing COVID19 mRNA vaccines. You can read more from the two press releases published here and here.

IUBMB Journal Highlights | June 2022

We are excited to highlight new research from the IUBMB Journals: IUBMB Life, BioFactors, Biotechnology and Applied Biochemistry, and Biochemistry and Molecular Biology Education.

Please also consider submitting your own research to the IUBMB Journals. You can expect to work with distinguished Editorial Board members and benefit from worldwide circulation and readership through our publishing partnership with Wiley. For more information about the journal and submissions, feel free to peruse the IUBMB journals website.

For now, please enjoy highlights of our recent content. Happy reading!

 


IUBMB Life

New Issue: Volume 74, Issue 6IUBMB life cover

Issue Highlights

β-Elemene induces apoptosis by activating the P53 pathway in human hypertrophic scar fibroblasts

Qin Guo, Yujia Li, Yuan Chen, Jin Ji, Shizhong Zheng, Xuefen Xu, Biyun Zhang, Jianzhou Ye

First published: 16 March 2022

Hypertrophic scar (HS) is a condition characterized by excessive synthesis and deposition of collagen. There are many clinical methods to alleviate HS, but most of them are accompanied by many complications. To investigate the effects of β-Elemene, extracted from the ginger family plant Wenyujin, on human hypertrophic scar fibroblast (hHSFs). Cultured hHSFs and human normal fibroblasts, observed the effect of β-Elemene on apoptosis, extracellular matrix, and endoplasmic reticulum stress (ERS) by western blot, Real Time-Polymerase Chain Reaction (RT-PCR), and flow cytometry. Based on our findings, it is clear that β-Elemene could inhibit the expression of α-smooth muscle actin (α-SMA), collagen I, and fibronectin, reduced collagen deposition. Further studies had found that β-Elemene could increase the expression of ERS-related proteins CHOP and Calnexin in a dose-dependent manner, thereby promoting the aggregation of cleaved-caspase-3 and inducing hHSFs to undergo poptosis. This process may depend on the regulation of P53. The results of our study indicates that β-Elemene induced hHSFs to undergo apoptosis though ERS pathway in a P53-dependent manner, which means that our research provided a new strategy for the development of drugs for the treatment of HS.

BSA

 

Induced effect of zinc oxide nanoparticles on human acute myeloid leukemia cell apoptosis by regulating mitochondrial division

Xuewei Yin, Zonghong Li, Chunyi Lyu, Yan Wang, Shumin Ding, Chenchen Ma, Jingyi Wang, Siyuan Cui, Jinxin Wang, Dadong Guo, Ruirong Xu

First published: 05 April 2022

Zinc oxide nanoparticles (ZnO NPs) have exhibited excellent anti-tumor properties; the present study aimed to elucidate the underlying mechanism of ZnO NPs induced apoptosis in acute myeloid leukemia (AML) cells by regulating mitochondrial division. THP-1 cells, an AML cell line, were first incubated with different concentrations of ZnO NPs for 24 hr. Next, the expression of Drp-1, Bcl-2, Bax mRNA, and protein was detected, and the effects of ZnO NPs on the levels of reactive oxygen species (ROS), mitochondrial membrane potential (Δψm), apoptosis, and ATP generation in THP-1 cells were measured. Moreover, the effect of Drp-1 inhibitor Mdivi-1 and ZnO NPs on THP-1 cells was also detected. The results showed that the THP-1 cells survival rate decreased with the increment of ZnO NPs concentration and incubation time in a dose- and time-dependent manner. ZnO NPs can reduce the cell Δψm and ATP levels, induce ROS production, and increase the levels of mitochondrial division and apoptosis. In contrast, the apoptotic level was significantly reduced after intervention of Drp-1 inhibitor, suggesting that ZnO NPs can induce the apoptosis of THP-1 cells by regulating mitochondrial division. Overall, ZnO NPs may provide a new basis and idea for treating human acute myeloid leukemia in clinical practice.

125OH2D3-treatment

 

Cytokeratin 13 promotes radiotherapy sensitivity of nasopharyngeal carcinoma by downregulating the MEK/ERK pathway

Ming Shi, Jia Wan, Huan Wang, Hong Yu

First published: 15 April 2022

Background

Radiation therapy is the first treatment choice for nasopharyngeal carcinoma (NPC), while radiation resistance and recurrence have become the primary factors and are associated with poor prognosis in the clinical treatment of NPC patients. The purpose of the present study was to explore the sensitivity and molecular basis of cytokeratin 13 (CK13) that regulates NPC radiotherapy.

Methods

HNE-3 or C666-1 cell line was used for overexpression and knockdown tests. Under radiotherapy conditions, CCK-8 assay, clone formation assay, and flow cytometry analyzed the effects of CK13 overexpression on cell proliferation, apoptosis, and cell cycle, respectively. In addition, Western blotting detected CK13-mediated downregulation of cell cycle-related genes. The mouse subcutaneous tumor-bearing experiment identified the effects of CK13 overexpression on the treatment of NPC in vivo. Further, Western blotting, CCK-8 assay, and flow cytometry investigated whether the CK13-mediated cell apoptosis involves the MEK/ERK signaling pathway.

Results

Overexpression of CK13 significantly inhibited the survival of HNE-3 cells under radiotherapy in vitro and in vivo, and there was a substantial decrease in cyclin-dependent kinase 4 and 6 (CDK4/6) levels promoting the cell percentage number in the G2/M phase and, subsequently, the ratio of the apoptotic cells. In contrast, the knockdown of CK13 showed the opposite partial regulatory effect. Interestingly, CK13 overexpression also showed a reduction in the survival of C666-1 cells and an increased ratio of the apoptotic cells under radiotherapy treatment. Furthermore, higher levels of CK13 downregulated the MEK/ERK signaling pathway, resulting in decreased HNE-3 cell proliferation and increased apoptosis. However, ERK activators were able to rescue the process partially.

Conclusions

Together, these results showed that CK13 promoted the radiosensitivity of NPC cells by downregulating the MEK/ERK signaling pathway. Thus, targeting CK13 provided insights into the treatment of NPC radiotherapy.

MER_ERK

 

SPECIAL ISSUE CALL FOR PAPERS

 

                                  Multicellular Microenvironment Effects on the Modulation of Cell Functions IUBMB life cover

GUEST EDITOR:   Xiangya Ding, Nanjing Medical University
 
Deadline extension for manuscript submission 30 June 2022
Expected issue publication will be November 2022

 

Extracellular Matrix: The Dynamic Structural and Functional Network in Health and Disease IUBMB life cover

GUEST EDITOR:   Xiangya Ding, Nanjing Medical University
 
Deadline extension for manuscript submission 30 June 2022
Expected issue publication will be November 2022

 

 

                                  Cancer drug resistance: molecular mechanisms, and therapeutic implications IUBMB life cover

GUEST EDITOR:   Mandeep Kaur, University of the Witwatersrand
 
Deadline extension for manuscript submission 30 September 2022
Expected issue publication will be summer 2023

 
 
 
 

NEW VIRTUAL ISSUES

See all the new IUBMB Life Virtual Issues here

 


BioFactors

New Special Issue: Volume 48, Issue 2BioFactors cover

Issue Highlights

Curcuma longa and curcumin affect respiratory and allergic disorders, experimental and clinical evidence: A comprehensive and updated review

Arghavan Memarzia, Saeideh Saadat, Sepideh Behrouz, Mohammad Hossein Boskabady

First published: 31 December 2022

Curcuma longa and its constituents, mainly curcumin, showed various of pharmacological effects in previous studies. This review article provides updated and comprehensive experimental and clinical evidence regarding the effects of C. longa and curcumin on respiratory, allergic, and immunologic disorders. Using appropriate keywords, databases including PubMed, Science Direct, and Scopus were searched until the end of October 2021. C. longa extracts and its constituent, curcumin, showed the relaxant effect on tracheal smooth muscle, which indicates their bronchodilatory effect in obstructive pulmonary diseases. The preventive effects of extracts of C. longa and curcumin were shown in experimental animal models of different respiratory diseases through antioxidant, immunomodulatory, and anti-inflammatory mechanisms. C. longa and curcumin also showed preventive effects on some lung disorders in the clinical studies. It was shown that the effects of C. longa on pulmonary diseases were mainly due to its constituent, curcumin. Pharmacological effects of C. longa extracts and curcumin on respiratory, allergic, and immunologic disorders indicate the possible therapeutic effect of the plant and curcumin on these diseases.

curcuma

 

Recent research progress of biologically active peptides

Linlin Kang, Tingting Han, Hailin Cong, Bing Yu, Youqing Shen

First published: 25 January 2022

With the rapid development of molecular biology and biochemical technology, great progress has been made in the study of peptides. Peptides are easy to digest and absorb, with lowering of blood pressure and cholesterol, improving immunity, regulating hormones, antibacterial, and antiviral effects. Peptides also have physiological regulation and biological metabolism functions with applications in the fields of feed production and biomedical research. In the future, the research focus of bioactive peptides will focus on their efficient preparation and application. This article introduces a comprehensive review of the types, synthesis, functionalization, and bio-related applications of bioactive peptides. For this aim, we introduced in detail various biopeptides and then presented the production methods of bioactive peptides, such as enzymatic synthesis, microbial fermentation, chemical synthesis, and others. The applications of bioactive peptides for anticancers, immune therapy, antibacterial, and other applications have been introduced and discussed. And discussed the development prospects of biologically active peptides.

active peptides

 

Nutrient-sensing amyloid metastasis

Luís Maurício T. R. Lima, Tháyna Sisnande

First published: 06 February 2022

Amyloids are organized suprastructural polypeptide arrangements. The prevalence of amyloid-related processes of pathophysiological relevance has been linked to aging-related degenerative diseases. Besides the role of genetic polymorphisms on the relative risk of amyloid diseases, the contributions of nongenetic ontogenic cluster of factors remain elusive. In recent decades, mounting evidences have been suggesting the role of essential micronutrients, in particular transition metals, in the regulation of amyloidogenic processes, both directly (such as binding to amyloid proteins) or indirectly (such as regulating regulatory partners, processing enzymes, and membrane transporters). The features of transition metals as regulatory cofactors of amyloid proteins and the consequences of metal dyshomeostasis in triggering amyloidogenic processes, as well as the evidences showing amelioration of symptoms by dietary supplementation, suggest an exaptative role of metals in regulating amyloid pathways. The self- and cross-talk replicative nature of these amyloid processes along with their systemic distribution support the concept of their metastatic nature. The role of amyloidosis as nutrient sensors would act as intra- and transgenerational epigenetic metabolic programming factors determining health span and life span, viability, which could participate as an evolutive selective pressure.

amyloids_1

 


Biotechnology and Applied Biochemistry

New Issue: Volume 69, Issue 2BAB cover

Issue Highlights

Pharmacological evaluation of the effects of enzymatically liberated fish oil on eosinophilic inflammation in animal models

Crawford Currie, Bomi Framroze, Dave Singh, Deepali Sharma, Christian Bjerknes, Erland Hermansen

First published: 30 March 2022

The inappropriate activation of eosinophils is a well-recognized driver of various human inflammatory diseases including asthma, chronic rhinitis, and various gastrointestinal diseases, including eosinophilic esophagitis. Steroids, both topical and systemic, remain a cornerstone of treatment and can be highly effective. However, some individuals suffer side effects, unresolved symptoms, or both. OmeGo, an enzymatically liberated fish oil, has demonstrated anti-inflammatory and antioxidant properties as well the reduction of the activation, migration, and survival of eosinophils. Two animal models of eosinophilic inflammation were used to further assess OmeGo’s profile. A house dust mite model of induced asthma showed a significant reduction in eosinophilic lung inflammation compared to the negative control, linoleic acid. The CRTH2 antagonist fevipiprant showed a similar eosinophilic inhibitory profile to OmeGo. In contrast, cod liver oil had no impact on any measure of inflammation. A guinea pig model of mild intraperitoneal eosinophilia showed a significant reduction in eosinophil activity by OmeGo, assessed by chemotaxis and chemokinesis. Apolipoprotein A-IV, an endogenous human protein with anti-inflammatory actions, showed a similar but numerically lower effect. OmeGo therefore combines a consistent antieosinophilic action with the known anti-inflammatory effects of polyunsaturated fatty acids. Proof-of-concept studies in asthma are warranted.

pharmacological evaluation

 


Biochemistry and Molecular Biology Education

New Virtual Issue on Teaching in the Time of COVID-19Biochemistry and Molecular Biology Education

Volume 50, Issue 3

Issue Highlights

Using e-learning tools in biochemistry teaching for undergraduate medical students in multicultural environment in Ukraine during COVID-19 crisis

Svitlana Volodarets, Nataliia Chernousova, Hanna Peleshenko ,Hanna Maslak, Anastasiia Savchenko, Olha Netronina

First published: 01 April 2022

Through the special situation like the COVID-2019 lockdown, interactive techniques play a prime role in the study process. The strategy of lockdown ways of higher education was provided in Ukraine as well as in other countries since March 2020. Our investigation was performed at the Department of Biochemistry and Medical Chemistry of Dnipro State Medical University during the first lockdown of COVID-19 from March to June 2020. The main aim of the research was the evaluation of the students’ attitudes to studying Biochemistry using Google classrooms and Moodle. Four hundred and sixty-three students studying in English, French, and Ukrainian took part in the survey. Our results reveal that majority of the students found Google classrooms and Moodle as useful e-learning tools. However, questions dealing with the technical issues of using the Google classrooms on laptops and mobile phones and submitting the replies to the Google classrooms cause difficulties among the students.

e-learning

 

Teaching the process of science through COVID-19 pandemic themes

Meghan Ward, Fiona Rawle

First published: 16 March 2022

Several predominant themes have emerged during the COVID-19 global pandemic that intersect with the nature and process of science. This paper identifies three such themes and briefly explores how they can be used as case studies and narrative cornerstones in teaching and learning. The themes include: (1) the understanding that science is cumulative and ever-changing, meaning that new findings may cause us to reconsider previous understandings; (2) the importance of citation tracking in the process of science; and (3) the need for accessible and purposeful science communication.
 

 


Did you know? Wiley and Jisc just signed an agreement that allows UK authors to publish Open Access in the IUBMB Journals at no cost to them.

Thanks to a partnership our publisher Wiley has signed with Jisc, certain UK institutions now have full access to journals published by Wiley, including the IUBMB Journals. Further, the partnership enables authors at participating UK institutions to publish open access at no cost to them in the IUBMB Journals. Payment of the associated Article Publication Charges (APC) would be covered via the partnership, and authors will not need to cover the APCs from their own pockets.

Wiley has also signed similar agreements with universities in Germany, the Netherlands, Austria, Norway, Hungary, Finland, Sweden, and with the US-based OhioLink And VIVA.

Submit your research to the IUBMB Journals today.


Molecular Aspects of Medicine

Molecular Aspects of Medicine cover

 
Volume 82 (December 2021) 100973
 
Autophagy in liver diseases: A review
 
by Qian, Huia; Chao, Xiaojuan; Williams, Jessicaa; Fulte, Sam; Li, Tiangang; Yang, Ling; Ding, Wen-Xing

 

Should you have a proposal or an idea for a thematic issue for Molecular Aspects of Medicine, please complete the thematic issue proposal form and send it to Dr. Angelo Azzi (angelo.azzi@tufts.edu)

 

Congratulations to our IUBMB Plenary Lecturers of June 2022

IUBMB Plenary Award Lecture_John Scott

Congratulations to Professor John Scott from the University of Washington School of Medicine, US, who will be presenting the IUBMB Plenary Award Lecture at the Gordon Research Conference: Cyclic Nucleotide Phosphodiesterases on “Exploring and Exploiting the Spatial Constraints of cAMP Signaling”

 

IUBMB Plenary Award Lecture_KRISTIJAN RAMADAN

Congratulations to Professor Kristijan Ramadan from Oxford Institute for Radiation Oncology, Department of Oncology, University of Oxford, UK who will be presenting the IUBMB Plenary Award Lecture at HDBMB22: From Science to Knowledge on “DNA-protein crossslink proteolysis repair: from human disease to cancer therapy”

2022 April_Travel Fellowship

Congratulations to our IUBMB Travel Fellows of April 2022

CONGRATULATIONS to our latest Fellows, from Brazil, Chile, South Africa, and UK, who have received fellowships to travel and attend a meeting in the IUBMB region.

 
Travel Fellowship_Jasmin Aschenbrenner
 
Travel Fellowship_Jasmin Aschenbrenner
Travel Fellowship_Karolina Brzezinska
 
Travel Fellowship_Karolina Brzezinska
Travel Fellowship_Juan Gonzalez
 
Travel Fellowship_Juan Gonzalez
Bruno Rodrigues_Travel Fellowship
 
Bruno Rodrigues_Travel Fellowship
Leslye Venegas_Travel Fellowship
 
Leslye Venegas_Travel Fellowship

2022 FEBS-IUBMB-ENABLE Conference

Dear PhD students and postdoctoral fellows,

We are very delighted to invite you to the 1st FEBS-IUBMB-ENABLE conference entitled “The perfect tandem: How technology expands the frontiers of biomedicine”, which will take place in Seville, Spain on 16-18th November 2022.

 
FEBS-IUBMB-ENABLE_sign

The FEBS-IUBMB-ENABLE conference is an international, interdisciplinary three-day event entirely organized by and for young researchers –PhD students and postdocs– from the molecular life sciences disciplines, bringing together Biomedicine and Technology. The FEBS-IUBMB-ENABLE conference series is a joint initiative of the Federation of European Biochemical Societies (FEBS), the International Union of Biochemistry and Molecular Biology (IUBMB) and four leading biomedical research institutes across Europe: the Institute for Research in Biomedicine – IRB (Barcelona, Spain), the Radboud Institute for Molecular Life Sciences – RIMLS (Nijmegen, The Netherlands), the Novo Nordisk Foundation Center for Protein Research – NNF CPR (Copenhagen, Denmark), and the European School of Molecular Medicine – SEMM (Milan, Italy), together with the Institute of Biomedicine of Seville – IBiS (Seville, Spain), where the 2022 event will be held.

Registration for this event will be open Wednesday, June 1st 2022. Early bird discounts are available until July 29th, 2022. A considerable number of travel grants are available for participants who actively participate via poster presentation or a short talk. The deadline to apply for travel grants is also July 29th, 2022.

Join us for three days of FEBS-IUBMB-ENABLE 2022 and have the opportunity to participate in:

Scientific symposium

Two full days ( 16th & 17th) during which young scientists from all over Europe will learn about different biomedical fields and cutting-edge techniques through scientific talks and informal chats with renowned scientists. Also, the scientific symposium provides an excellent platform for young researchers to present their own work in short talks and posters, which are selected from the abstracts. Participants will also have the chance to talk to speakers during the gala dinner on Thursday, 17th November.

Outreach activities

An important part of ENABLE is focused on bridging the gap between scientists and society. To this end, FEBS-IUBMS-ENABLE organizes outreach activities that allow participants to explain biomedicine to the general public and bring science closer to society. Particularly the pub talks on Wednesday, 16th November, organized in pubs in the city of Seville, are an excellent opportunity for young researchers to bring their science to the public.

Science career day

A full day (Friday, 18th November) during which participants will be able to attend workshops and career chats with professionals. Also, participants will have the chance to learn about career opportunities at the “Job Fair”, where various companies from different sectors (publishing, biotech, pharma) will be present.

To encourage the engagement of participants, FEBS-IUBMS-ENABLE has also planned an optional Seville city tour and a visit to the science museum the evening before the conference (Tuesday, 15th November). Additionally, this year a scientific photography contest will be run throughout the conference, and there will be prizes for the best and the favorite photos.

For more information, visit our website, where you can also watch videos of previous editions and remember to follow us on Instagram, LinkedIn, Facebook and Twitter!

We look forward to meet you in Seville,
Sincerely,
the Scientific Organizing Committee of FEBS-IUBMB-ENABLE 2022

WW Fellowship_2022 April recipients

Congratulations to our IUBMB Fellows of April 2022

CONGRATULATIONS to our latest Fellows, from Argentina, Brazil, Cameroon, Cuba, India, Mexico, Norway, and Portugal, who have received fellowships to travel to a lab in a different country for 4 months. We wish them much success in their research!

WW Fellowship_2022 April_Danielle Cozachenco
 
WW Fellowship_2022 April_Danielle Cozachenco
WW Fellowship_2022 April_Ines Vieira da Silva
 
WW Fellowship_2022 April_Ines Vieira da Silva
WW Fellowship_2022 April_Claudia Diaz Borges
 
WW Fellowship_2022 April_Claudia Diaz Borges
WW Fellowship_2022 April_Geronimo Marcelo Ducca
 
WW Fellowship_2022 April_Geronimo Marcelo Ducca
WW Fellowship_2022 April_Khadija El Jellas
 
WW Fellowship_2022 April_Khadija El Jellas
WW Fellowship_2022 April_Romina Celeste Geysels
 
WW Fellowship_2022 April_Romina Celeste Geysels
WW Fellowship_2022 April_Fernando Guzman-Chavez
 
WW Fellowship_2022 April_Fernando Guzman-Chavez
WW Fellowship_2022 April_Harsh Kumar
 
WW Fellowship_2022 April_Harsh Kumar
WW Fellowship_2022 April_Florencia Malizia
 
WW Fellowship_2022 April_Florencia Malizia
WW Fellowship_2022 April_Lucas Servi
 
WW Fellowship_2022 April_Lucas Servi
 
 
IUBMB Journals

IUBMB Journal Highlights | May 2022

We are excited to highlight new research from the IUBMB Journals: IUBMB Life, BioFactors, Biotechnology and Applied Biochemistry, and Biochemistry and Molecular Biology Education.

Please also consider submitting your own research to the IUBMB Journals. You can expect to work with distinguished Editorial Board members and benefit from worldwide circulation and readership through our publishing partnership with Wiley. For more information about the journal and submissions, feel free to peruse the IUBMB journals website.

For now, please enjoy highlights of our recent content. Happy reading!

 


IUBMB Life

New Issue: Volume 74, Issue 4IUBMB life cover

Issue Highlights

Evaluating the therapeutic efficacy of radiolabeled BSA@CuS nanoparticle-induced radio-photothermal therapy against anaplastic thyroid cancer

Chunmei Zhang, Jinyan Chai, Qiang Jia, Jian Tan, Zhaowei Meng, Ning Li, Menghui Yuan

First published: 03 February 2022

Bovine serum albumin (BSA) has been employed as a mild biological template in nanoscale particles. Copper sulfide (CuS) has been used for photothermal therapy (PTT) in several studies. In this study, we aimed to synthesize the 131I-labeled BSA-modified CuS nanoparticles (131I-BSA@CuS), with attributes of both radiotherapy and PTT, as a therapeutic agent against anaplastic thyroid carcinoma (ATC). BSA@CuS nanoparticles were prepared using the solvothermal reaction and then labeled with Na131I by the chloramine-T method. The products were characterized and their cytotoxicity was investigated in vitro and in vivo. The therapeutic efficacy of 131I-BSA@CuS was evaluated in ARO cell (an ATC cell line) subcutaneous tumors. The nanoparticles showed good biocompatibility and low toxicity in vitro and in vivo. BSA@CuS rapidly and effectively converted the light energy from an 808 nm laser into thermal energy with a conversion efficiency of 28.07%. SPECT/CT imaging demonstrated that the accumulation of radioactivity peaked within 24 hr and resided in the tumors for 5 days post intratumoral injection. In vivo assays indicated that, compared to monotherapy, the synthesized nanoparticles employing both PTT and radiotherapy possess better therapeutic efficacy against tumors. The synthesized nanomaterial showed uniform dispersion, good stability and aqueous solubility, excellent photothermal properties, and long-term retention in ATC. Hence, combined radiotherapy and PTT can significantly inhibit tumor growth compared to monotherapy, and can be applied in clinical settings.

BSA

 

The effects of 1,25(OH)2D3 treatment on immune responses and intracellular metabolic pathways of bone marrow-derived dendritic cells from lean and obese mice

Da Hye Cho, Ga Young Lee, Jeong Hee An, Sung Nim Han

First published: 28 December 2021

Vitamin D affects differentiation, maturation, and activation of dendritic cells (DCs). Obesity-related immune dysfunction is associated with metabolic changes in immune cells. Objectives of the study are to investigate the effects of vitamin D and obesity on immune responses and markers related to immunometabolism of bone marrow-derived dendritic cells (BMDCs). Bone marrow cells (BMCs) were isolated from lean and obese mice, and BMDCs were generated by culturing BMCs with rmGM-CSF. BMDCs were treated with 1 or 10 nM of 1,25-dihydroxyvitamin D3 (1,25(OH)2D3), and maturation was induced by LPS (50 ng/ml) stimulation for 24 hr. Cell phenotypes, cytokine productions, and expression of proteins and genes involved in Akt/mTOR signaling pathway and glycolytic pathway were determined. 1,25(OH)2D3 treatment inhibited differentiation of BMDCs (CD11c+ %), expression of phenotypes related with DC function (MHC class II and CD86) and production of IL-12p70 in both lean and obese mice. The expression of PD-L1 and the ratio of IL-10/IL-12p70 were increased by 1,25(OH)2D3. With 1,25(OH)2D3 treatment, Akt/mTOR signaling pathway was suppressed, and expression of genes related to glycolysis (Glut1, Pfkfb4, and Hif1A) was increased. The upregulation of glycolysis-related genes observed with 1,25(OH)2D3 treatment seems to be associated with the induction of tolerogenic features of BMDCs from lean and obese mice, and Hif1A seems to have a potential role in conveying the effect of 1,25(OH)2D3 on glycolysis.

125OH2D3-treatment

 

KLHL38 facilitates staurosporine-induced apoptosis in HL-1 cells via myocardin degradation

Ying Luo, Lei Tian, Chen Liang, Yao Xu

First published: 03 February 2022

Cardiac apoptosis has been identified as one of the main precipitating factors of heart failure (HF) throughout the whole course of progressive disease. Limited to the lack of diagnostic markers and effective drug targets, cardiac apoptosis is still a major clinical challenge. Here, we reveal a potential novel therapeutic target for cardiac apoptosis. In the cause of the study, we found that KLHL38 was highly expressed in cardiac tissue of HF patients via GEO data-mining, which was further verified in the heart tissue of transverse aortic constriction mice. Meanwhile, the expression of KLHL38 is negatively correlated with myocardin protein level, which is a key cardiac apoptosis regulator. The KLHL38 overexpression obviously promoted cardiomyocyte apoptosis treated with staurosporine by facilitation of myocardin’s ubiquitylation and subsequent proteasomal degradation. These findings reveal a new therapeutic target, which may provide a new theoretical foundation for the treatment of myocardial apoptosis in clinical practice.

125OH2D3-treatment

 

SPECIAL ISSUE CALL FOR PAPERS

 

                                  Long non-coding RNAs: sequence, structure, function and evolution IUBMB life cover

GUEST EDITORS:   Toni Gabaldón, Barcelona Supercomputing Center and Institute for Research in Biomedicine, Spain; Lovorka Stojic, Barts Cancer Institute, QMUL, UK; Uciel Chorostecki, Barcelona Supercomputing Center and Institute for Research in Biomedicine
 
Manuscripts should be submitted by 31 May 2022
Expected issue publication will be September 2022
 

 
                                  Multicellular Microenvironment Effects on the Modulation of Cell Functions IUBMB life cover

GUEST EDITOR:   Xiangya Ding, Nanjing Medical University
 
Deadline extension for manuscript submission 30 June 2022
Expected issue publication will be November 2022

 

Extracellular Matrix: The Dynamic Structural and Functional Network in Health and Disease IUBMB life cover

GUEST EDITOR:   Xiangya Ding, Nanjing Medical University
 
Deadline extension for manuscript submission 30 June 2022
Expected issue publication will be November 2022

 
 
 
 

NEW VIRTUAL ISSUES

See all the new IUBMB Life Virtual Issues here

 


BioFactors

BioFactors coverNew Special Issue: Volume 48, Issue 2

Issue Highlights

Maternal erythrocyte fatty acid composition as a predictive marker for pregnancy health

Shabnam Fayezi, Amir Mehdizadeh, Ariane Germeyer, Thomas Strowitzki, Parisa Fayyazpour, Zahra Nowrouzi, Reza Zarezadeh

First published: 31 March 2022

Pregnancy is accompanied by a surge in demand for fatty acids (FAs) in order to support maternal health, as well as fetal growth and development. Of particular demand is essential for long-chain polyunsaturated FAs. FAs are primarily obtained from dietary sources and are distributed in the body. In comparison with the use of self-reporting approaches, measuring the FA levels within different blood compartments can present a more accurate image of nutritional, and thus tissue, FA composition. Hence, the FA profile of plasma or serum is commonly used for physiological analyses. Nevertheless, plasma and serum FAs are not yet incorporated into cell membranes, and consequently may not be a suitable reflection of the FA status of body tissues. The evaluation of erythrocyte FA levels offers a superior possibility for the following reasons: the biological fluctuation of erythrocyte FA composition is low, phospholipids account for almost all the lipid content of erythrocytes, and the FA profiles of erythrocytes represent those of tissues. Here, we elaborate on whether the status of maternal erythrocyte FAs can serve as a prognostic biomarker for reproductive health and fetomaternal complications, including embryonic and fetoplacental development, gestational length, and preeclampsia. In addition, factors with the potential of altering the maternal erythrocyte FAs such as maternal diet, lifestyle habits, genetics, and body composition are discussed.

Paclitaxel

 


Biotechnology and Applied Biochemistry

Biotechnology and Applied Biochemistry coverVolume 69, Issue 2

Issue Highlights

An electrochemical aptasensor for detection of prostate-specific antigen-based on carbon quantum dots-gold nanoparticles

Mehrab Pourmadadi, Alireza Nouralishahi, Mohammad Shalbaf, Javad Shabani Shayeh, Amideddin Nouralishahi

First published: 19 March 2022

In this work, an electrochemical aptasensor was described for the determination of prostate-specific antigen (PSA). Aptamer chains were decorated on the surface of a glassy carbon electrode (GCE) via carbon quantum dots/Au nanoparticles (Au/CQD). Structural analysis that was used to characterize the prepared materials shows that Au/CQD nanoparticles synthesized in a spherical shape with an average size of 70 nm. Furthermore, the combination of Au nanoparticles with CQD resulted in formation of crystalline the structure of the Au/CQD composite. To study the electrochemical performance of the prepared aptasensor, cyclic voltammetry, square wave voltammetry, and electrochemical impedance spectroscopy were used. The results show that the aptasensor has a good selectivity to PSA over other biomaterials with the time optimized about 30 min. K4[Fe(CN)6] was used as an electrochemical probe with the limit of detection about 2 fg⋅mL–1. To avoid the hazardous nature of K4[Fe(CN)6], a label-based aptasensor was prepared using methylene blue as an electrochemical signal producer. They provide the capability of electrochemical detection in buffer phosphate solution with high sensitivity.

aptasensor

 


Biochemistry and Molecular Biology Education

New Virtual Issue on Teaching in the Time of COVID-19Biochemistry and Molecular Biology Education

Volume 50, Issue 2

Issue Highlights

It is all about the students…

Joseph J. Provost

First published: 20 April 2022

Being nominated and then recognized by the American Society for Biochemistry and Molecular Biology’s (ASBMB) award for “Exemplary Contributions to Education” (https://www.asbmb.org/career-resources/awards-grants-fellowships/education) is an incredible honor. The people who have previously been bestowed with this accolade are impressive and have done extraordinary things. Each prior awardee has moved the needle to advance the education of undergraduate, graduate, or medical students beyond their individual classrooms and laboratories. However, understanding how I might be worthy of this award is a real struggle when encapsulating what I have done to earn this honor. Oh, I recognize that I have done many things, been involved in several groups, and been fortunate to be asked to serve on and volunteered to be on more than a few committees that required a serious amount of service. However, this does not make an award-winning moment. The unifying factor—what all of this work has been “it is about the students.”
 

Teaching the “acid–base” subject in biochemistry via virtual laboratory during the COVID-19 pandemic

Filiz Avcı

First published: 20 April 2022

Virtual laboratories have started to be the leading alternative teaching tools during the Covid-19 pandemic process. The “Acid–Base” subject is among those that form the foundations of biochemistry. Students can learn the “Acid–Base” subject in a secure environment, with remote access through the use of virtual laboratory simulations. Simulation was applied to fourth-year undergraduate students in Turkey who want to obtain a bachelor’s degree in science teaching during the present study. During the application process, the students conducted different experiments on the concepts of strong acid, strong base and pH, which are the basic concepts of biochemistry. In addition, student opinions regarding the application were also obtained. It was thus determined that the majority of the students were able to write hypotheses, test their accuracy and report the test findings correctly; a small number of students started to apply the simulations after examining them in accordance with scientific terminology. At the end of the application, the students also stated that they had fun during the application which also provided effective learning. It is recommended that classroom activity which is integrated with simulation be used in Chemistry, Biochemistry and Science Laboratory Applications courses.
 

acid base

 


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Molecular Aspects of Medicine

Molecular Aspects of Medicine cover

 
Volume 82 (December 2021) 100973
 
Autophagy in liver diseases: A review
 
by Qian, Huia; Chao, Xiaojuan; Williams, Jessicaa; Fulte, Sam; Li, Tiangang; Yang, Ling; Ding, Wen-Xing